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Faculty - Dr. Mark Bolyard

Chair, Biology Department
138 White Hall, Box 3021
Union University
Jackson, Tennessee 38305
(731) 661-6586

Biology Chair Questionnaire


  • B. A., Biology and Secondary Certification in Biology and Chemistry: Hanover College, Hanover, Indiana, May 1984.
  • Ph. D. in Experimental Pathology: School of Medicine, University of North Carolina, Chapel Hill, May 1989.

Previous Experience

  • Research Associate, Plant Molecular Biology, Michigan State University , 1989-1991.
  • Faculty Member of Biological Sciences, Southern Illinois University, Edwardsville, 1991-2006

Courses Commonly Taught

  • BIO 100: Survey of Biological Concepts
  • BIO 110: Global Biology
  • BIO 323: Cell Biology
  • BIO 325: Molecular Biology
  • BIO 498: Senior Seminar

Research/Scholarship Interests

  • Development of novel plant tissue culture procedures
  • Production of recombinant proteins and peptides in E. coli

Current undergraduate research projects supervised

  • Kevin Luy: Evaluation of growth regulator concentration reduction in African mahogany
  • Nathan Peace: Modification of the GFP protein to contain the 6 x His affinity tag
  • Will Tucker: Modification of the GFP protein to contain the streptactin affinity tag

Completed undergraduate research projects supervised

  • Robyn Reynolds: Evaluation of the timing of auxin removal in the regeneration of African mahogany.
  • Brandon Churchill: Formation of an indicator for thrombin inhibitors using recombinant green fluorescent protein (with Dr. Marc Lockett)
  • Joseph Dresser: Integration of a Factor Xa recognition sequence into pGLO plasmid to synthesize a modified green fluorescent protein from Escherichia coli cells (with Dr. Marc Lockett)
  • Joshua Brinkman: Addition of affinity tags for the purification of the green fluorescent protein
  • Teala Terrell: Identification and Long-Term Storage of Bacteria and Fungi Induced by Gibberellic Acid in Populus Tremula Axenic Shoot Tip Cultures (with Mrs. Cathy Huggins)
  • Liane Gozmao: Introduction of multiple FXa or thrombin recognition sequences into the Green Fluorescent Protein at unique restriction sites (with Dr. Marc Lockett).
  • Travis Shutt: Introduction of FXa or Thrombin recognition sequences using PCR directed mutagenesis (with Dr. Marc Lockett).
  • Danielle Blackstone: Regeneration of African and Cuban Mahoghany from leaf tissue.
  • Callie Bodnarchuk: Expression of human secretin as a fusion with Green Fluorescent Protein inE. coli.
  • Hannah Henson: Insertion of a thrombin recognition sequence into Green Flourescent Protein (with Dr. Marc Lockett).
  • David Koh: Evaluation of thidiazuron, kinetin, and zeatin on regeneration of African mahogany.
  • Mary Kathryn LaMar: The effect of multiple acoustic frequencies on the folding of Green Fluorescent Protein.
  • Dustin Mayfield: Insertion of a factor Xa recognition sequence into Green Fluorescent Protein (with Dr. Marc Lockett).
  • Carrie Moore: Identification of endogenous bacteria in shoot tip cultures of European Aspen (with Mrs. Cathy Huggins).
  • Lauren Parlow: Rooting of regenerated shoots from Southern Wormwood, Artemesia abrotanum L.
  • Brandon Lowe: Site-directed mutagenesis of the Green Fluorescent Protein to restore fluorescence in the periplasmic space of E. coli.
  • Melissa Ratliff: Plant pectin as an alternative source of arabinose for GFP production in E. colivia Erwinia chrysanthemi pectinase activity.
  • Carson Rider: Evaluation of the Green Fluorescent Protein containing FXa or Thrombin recognition sequences (with Dr. Mark Lockett).
  • Jordann Staples: Callus formation from leaf tissues of African mahogany; examination of cytokinins and media.
  • Tsegayesus Temtem: Optimization of Secretion of Green Fluorescent Protein from E. coli.


Recent Presentations

  • "The Journey to Developing and Implementing an Interdisciplinary Humanitarian Studies Minor" presented at the Undergraduate Educators for Global Engagement conference, April 12-15, 2012.
  • "Stem Cells and Human Cloning", presentations to Latvian physicians (June 2010), Latvian medical students (June 2011), and Lithuanian Medical Students (June 2011).
  • "Integrating Teaching and Scholarship" presentation at the Faith-Filled Leadership Institute, June 14-17, 2008, Gordon College.

Recent Publications

  • "A Christian in the Sciences" chapter in Faith and Learning, David Dockery ed., 2102, Broadman and Holman, pp345-367, ISBN 978-1-4336-7311-5
  • "Integration of Teaching and Research Revisited" Mark Bolyard, Council on Undergraduate Research Quarterly, Fall, 2009 (Vol 30 [1]: 35-38)
  • "Integrating Research into a Large Teaching Laboratory" Joshua I.T. Jones and Mark Bolyard, Council on Undergraduate Research Quarterly, Fall, 2009 (Vol 30 [1]: 39-43)
  • "A Suggestion for Chairs Looking for Time to do Research" Mark Bolyard, The Department Chair, Fall, 2009 (Vol 20 [2]: 18-20)
  • "Allelopathic activity of a testa-derived solution from Siberian Maple (Acer ginnala Maxim.) seeds", John Cawly, Sara Newton and Bolyard, M., Allelopathy Journal 16:227-238, 2005.
  • "Expression and in vitro amidation of recombinant FMRFamide" Ian Grimes, Jason Aleman, Kevin Griffin, Betty Lawton, Tiffany Slater, Kevin Krajniak, and Mark Bolyard, Transactions of the Illniois State Academy of Sciences 97:23-31, 2004.
  • ’Lonesome Ginkgo’ lab: Embryo tissue culture from trees’, Mark Bolyard and Vicki Gillespie. The American Biology Teacher, 63:1-5, 2001.
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